Value of determination of mononuclear blood cell proliferation in vitro in the diagnosis of lymphosarcoma
Rupniewska, Z.M.; Rozynkowa, D.; Kowalewski, J.; Nowakowski, A.
Acta Haematologica Polonica 6(3): 185-190
1975
ISSN/ISBN: 0001-5814 PMID: 1174277 Document Number: 92271
The authors describe mitotic activity in non-stimulated cultures of mononuclear cells obtained from the peripheral blood of patients with lymphosarcoma. The investigations were carried out on 3 patients in stage 3rd and 4th of this disease and 5 patients in the stage of sarcomatous-cell leukaemia. During a period of 24-48 hours after setting up of culture a rise was observed in the number of blast cells and appearance of up to 10% of mitotic figures. This observation may be useful for differentiation of sarcomatous-cell leukaemia from chronic lymphocytic leukaemia in which cells obtained from the peripheral blood and cultured in vitro without addition of mitogen do not demonstrate mitotic activity.