A method of continuous recording on microsamples of the Hb-O2 association curve. II. A study of Bohr effect and carbamino-formation
Teisseire, B.; Teisseire, L.; Hérigault, R.; Soulard, C.
Bulletin de Physio-Pathologie Respiratoire 11(6): 853-862
1975
ISSN/ISBN: 0007-439X PMID: 7333 Document Number: 91345
The authors have worked out an adaptation to microsamples (200-400 mul) of the DUVELLEROY et al. method, allowing the continous registration of the O2-Hb association curve. The microsample being diluted in buffer solution, it is possible to predetermine its pH and PCO2. The prefixed conditions are maintained during the initial deoxygenation phase, and all along the curve registration. Moreover, the adjustment to the desired values of the pH and PCO2 allows the quantification of total BOHR effect, proton BOHR effect and carbamino-formation, during the course of oxygenation.