Lipogenesis and glyceride synthesis in the rat: response to diet and exercise
Askeq, E.W.; Dohm, G.L.; Doub, W.H.; Husotn, R.L.; Van Natta, P.A.
Journal of Nutrition 105(2): 190-199
1975
ISSN/ISBN: 0022-3166 PMID: 1113199 Document Number: 90654
The responses of hepatic and adipose tissue malic enzyme (ME), citrate cleavage enzyme (CCE), glucose-6-phosphate dehydrogenase (G6PD) and glyceride synthetase (GS) to exercise training and exhausting exercise and the potential of a high-carbohydrate diet to modify those responses were studied in male Carworth rats of bodyweight about 110 g. The high-carbohydrate diet increased and the high-fat diet diminished ME, CCE and G6PD. GS was decreased in skeletal muscle, liver and adipose tissues of the rats on the high-carbohydrate diet. A significant two-way diet-training interaction was indicated for hepatic ME and G6PD. That interaction resulted from an apparent training modulation of ME and G6PD responses to the high-fat and high-carbohydrate diets. Adipose tissue G6PD was significantly decreased by training. Exhausting exercise immediately before death did not significantly alter ME or CCE activities in either liver or adipose tissue, but decreased adipose tissue G6PD in untrained rats. Exhaustion was also associated with decreased GS activity in muscle and liver. Physical training was associated with a significant increase in GS in muscle and adipose tissues. In contrast to glyceride synthesis, no increase in adipose tissue lipogenic potential was noted in response to training, indicating that the physically trained rats may have greater ability to store but not to synthesize fatty acids.