Detection of pathogenic leptospira in the waste water and sewage sludge of large pig breeding sites
Mînzat, R.M.; Tomescu, V.
Archiv für Experimentelle Veterinarmedizin 29(4): 557-562
1975
ISSN/ISBN: 0003-9055 PMID: 1103773 Document Number: 88691
Sewage effluent and sludge from the purification plant of 8 large piggeries was examined for the presence of pathogenic leptospires. By using the methods of Appelman and Van Thiel it was found that 43.1% of samples of effluent were contaminated with L. pomona and O. tarassovi. Altogether 33 strains of pomona and three mixed cultures of pomona and tarassovi were obtained. The isolated strains were shown to be pathogenic by experimental infection of guinea-pigs, rabbits and pregnant and non-pregnant sows. The average period of survival of pathogenic leptospires in sewage effluent was 24 to 48 hours, with a maximum of 96 hours. Leptospires were killed within 24 hours in decanted sludge, owing to its strong acidity.