Solubilization of sarcolemma acetylcholinesterase with Triton X-100 and KCl
Dyadyusha, H.P.; Sanina, O.L.
Ukrains'kyi Biokhimichnyi Zhurnal 47(2): 172-178
1975
ISSN/ISBN: 0041-610X PMID: 1209759 Document Number: 87119
It is shown that simultaneous treatment with sarcolemma with triton X-100 (0.1-1.0%) and KCl (0.6-1.2 M) solubilizes about 60% of acetyl cholinesterase, however its specific activity in the intact sarcolemma and in the obtained fractions is the same. A considerable part of lipids (about 90% of phospholipids and 50% of cholesterol) may be removed by treating sarcolemma with 0.1% triton X-100; it may be fixed in the membrane by hydrophoric bonds. Other phospholipids and cholesterol are bound with sarcolemma proteins more strongly and, probably, form the protein-lipid complexes. In the composition of phospholipids strongly bound with sarcolemma proteins a relative content of sphingomyelin increases and that of lysolecithin decreases.