Enzymatic breakdown of steroids during 11 alpha- and 11 beta-hydroxylation of the 21-acetate of Reichstein's "substance S" by Tieghemella orchidis (fam. Mucoraceae)
Mogil'nitskiĭ, G.M.; Andreev, L.V.; Koshcheenko, K.A.
Mikrobiologiia 44(2): 351-356
1975
ISSN/ISBN: 0026-3656 PMID: 1226150 Document Number: 86017
By-products of the transformation were studied, which were formed in the process of 11alpha and 11beta-hydroxylation of 21-acetate of the Reichstein substance "S" by the culture of Tieghemella orchidis. The following steroid derivatives, known before, were isolated: epihydrocortisone, 6beta-hydroxy derivative of the Reichstein substance "S", hydrocortisone, cortisone. The following steroid derivatives were isolated from the initial chloroform extract of the fermentation medium, and identified: prednisolone, androstendione, testololactone, and 1,2-dehydro derivatives of the Reichstein substance S" and its 21-acetate. The quantitative ratio between the products of transformation depended on the conditions of growth and transformation. These compounds seem to be intermediate metabolites in the course of the steroid destruction via the pathway common for fungi and involving the destruction of D ring of the steroid molecule.