L-Glutamate-glyoxylate aminotransferase in Lactobacillus plantarum
Galas, E.; Florianowicz, T.
Acta Microbiologica Polonica. Series B Microbiologia Applicata 7(4): 243-252
1975
ISSN/ISBN: 0567-7823 PMID: 5859 Document Number: 86016
Glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of Lactobacillus plantarum. Purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. The molecular weight of the enzyme estimated by Sepharose 4B gel filtration amounts to 37.000. Micaelis constants for glyoxylate and glutamate are corresponding to 6.25 X 10(-3) M and 2.75 X 10(-3) M, respectively. Optimal pH in phosphate and veronal buffers is 8.0 and optimal temperature 35--37 degrees C.