The role of serum factors in the acceleration by Freund's complete adjuvant of the growth of transplanted murine leukemic cells
Byfield, P.E.; Finklestein, J.Z.; Tittle, K.L.; Hsi, C.; Imagawa, D.T.
Cancer Research 35(2): 409-414
1975
ISSN/ISBN: 0008-5472 PMID: 1109806 Document Number: 84948
Attempted nonspecific immunotherapy led to acceleration rather than retardation of tumor growth. Mice given injections of Freund's adjuvant were more susceptible to transplanted syngeneic Gross virus-induced leukemic cells when Freund's complete adjuvant was administered i.p. 0 to 7 days before or 1 day after tumor; thereafter, the adjuvant had no effect. Two serum-mediated phenomeana were demonstrated in vitro: (a) sera from mice immunized with Freund's complete adjuvant and tumor facilitated killing of tumor cells by peritoneal exudate cells from nonimmune mice; (b) sera from all mice with progressive tumor blocked the cytotoxicity of a xenogeneic tumor-specific serum. Certain sera produced both effects. However, sera that either blocked or facilitated tumor killing in vitro had no effect on the growth in vivo of transplanted tumor cells.