Suitability of immune serums from different animal species for the preparation of conjugates against Aujeszky's disease virus
Chumchal, R.; Zuffa, A.; Grunert, Z.
Archiv für Experimentelle Veterinarmedizin 29(3): 357-368
1975
ISSN/ISBN: 0003-9055 PMID: 172039 Document Number: 84687
A comparison was made of immune serum from rabbits, sheeps, pigs and cattle. Pure IgG was prepared by the Rivanol sulphate method and immune electrophoresis. Optimum protein quality, conjugation time under various conditions and using two preparations of fluorescein isothiocyanate were studied. Conjugate purified by DEAE-Sephadex A-50 and concentrated to 1% protein by ultrafiltration was freeze-dried and then tested for activity and the degree of non-specific fluorescence. Evaluation by yield of immuno-electrophoretically pure IgG, conjugation time, antibody content, degree of non-specific fluorescence and the intensity of specific fluorescence before and after dilution showed that pig serum was the best, followed by cattle and sheep serum; rabbit serum was the least suitable for conjugate preparation.