Scale-up of AOAC method 3 for quantitation and isolation of aflatoxins in nuts and nut products for chemical and biological confirmation
DiProssimo, V.P.
Journal - Association of Official Analytical Chemists 57(2): 349-352
1974
ISSN/ISBN: 0004-5756 PMID: 4816505 Document Number: 77538
The official AOAC Method III for aflatoxins in peanuts and peanut products (Best Foods or BF method) has been adapted to a larger sample size (500 g), approaching the sample handling capacity of the 1 kg official AOAC Method I (FDA Contaminants Branch or CB method). The modified procedure (NY method) was used in place of the 1 kg CB method for obtaining quantitative assay results and for isolation of pure aflatoxin B1 for derivatization and bioassay. Two samples of naturally contaminated unshelled pistachio nuts and 4 peanut product samples were analyzed using the modified NY method and the results were compared with quantitative assays using official methods. Results were in excellent agreement with the official methods. The thin layer chromatographically pure aflatoxin B1 recovered using the modified procedure on various products was 39 to 57% of that calculated to be present in the samples, a favorable recovery compared to the 1 kg CB method (usually about 50%). All derivatizations carried out were successful (3 samples) and all biological confirmations were successful (2 samples were tested by the chick embryo bioassay for aflatoxin B1 toxicity).