Production and prevention of fatty liver in rats fed clofibrate and orotic acid diets containing sucrose
Novikoff, P.M.; Roheim, P.S.; Novikoff, A.B.; Edelstein, D.
Laboratory Investigation; A Journal of Technical Methods and Pathology 30(6): 732-750
1974
ISSN/ISBN: 0023-6837 PMID: 4835600 Document Number: 76503
Male Sprague-Dawley rats fed on a semipurified diet containing 60% sucrose and 0.25% ethyl chlorophenoxybutyrate (clofibrate) developed mild fatty liver; triglyceride in liver more than doubled, but in plasma it was lower than in controls on high-sucrose or chow diets without clofibrate. The addition of 1% orotic acid to the high-sucrose diet resulted in severe fatty liver; liver triglycerides were about 9 times the normal after 8 days on the diets and about 20 times by day 16; plasma triglyceride concentration fell and by day 8 the apoprotein beta component of plasma lipoproteins disappeared. When clofibrate and orotic acid were added simultaneously to the high-sucrose diet, fatty liver did not develop; liver triglyceride values were lower than in sucrose-fed controls until day 8, but on day 16 they were about equal; plasma triglyceride fell, but total plasma apoprotein beta concentrations remained normal.