Liquid chromatographic method for determination of patulin in apple juice
Ware, G.M.; Thorpe, C.W.; Pohland, A.E.
Journal - Association of Official Analytical Chemists 57(5): 1111-1113
1974
ISSN/ISBN: 0004-5756 PMID: 4414496 Document Number: 74297
The apple juice sample is extracted with ethyl acetate and the extract is cleaned up by elution from a silica gel column with ethyl acetate-benzene. Patulin is determined by use of a liquid chromatograph, a 25 cm Zorbax-Sil column, and a 254 nm ultraviolet detector. This method has a lower detection limit of 11 μg/L with recoveries of patulin added to apple juice >85%. Gas chromatographic-mass spectrometric analysis of the acetate derivative is used for confirmation. Patulin was detected at levels ranging from 44 to 309 μg/kg in 8 of 13 commercial samples of apple juice.