Short-term exposure health effect of chromium in drinking water
Duan, L.; Gu, W.; Wang, C.; Shi, Y.; Wang, C.; Xu, Y.; Zhang, H.
Wei Sheng Yan Jiu 48(2): 295-302
2019
ISSN/ISBN: 1000-8020 PMID: 31133111 Document Number: 699673
To verify the health effect and the point of departure(POD) of chromium in drinking water by animal experiment, and acquire the short-term health advisory(HA). A total of 120 SPF SD rats were randomly divided into 6 groups. Deionized water was used as a negative control and the concentration of chromium was 0, 7. 2, 8. 3, 14. 4, 24. 0, 28. 8 mg/(kg·d) and the potassium chromate(K_2CrO_4) was used as a positive control. Blood analysis, blood biochemical parameters and histopathology were determined after exposure 14 and 28 days. The results were analyzed by one-way ANOVA, P<0. 05 showed that the difference was significant. After 28 days of chromium exposure, the liver weights of the female rats in each dose group were lower than the control group. There were significant differences between the 24. 0, 28. 8 mg/(kg·d) dose groups with the control group. The liver weight of female rats in the 24. 0, 28. 8 mg/(kg·d) groups were(6. 68±0. 90)g and(7. 08±0. 36)g, respectively, which were significantly higher than those in the control group(P<0. 05). After 28 days exposure, alanine transaminase(ALT) in male rats at 24. 0 and 28. 8 mg/(kg·d) levels were(59. 04±10. 98)U/L and(63. 78±5. 89)U/L, respectively, which were significantly higher than those in the control group(P<0. 05). Alkaline phosphatase(ALP) levels in blood of the two groups were lower than those in the control group, that were(130. 52±23. 22)U/L and(126. 34±28. 25)U/L(P<0. 05). With index of rat liver damage(liver weight, ALT, ALP) as indicators of health effect, LOAEL is 24 mg/(kg·d), and NOAEL is 14. 4 mg/(kg·d). Referring to the calculation method of HA value formulated by the United States Environmental Protection Agency(USEPA), the HA is 1. 44 mg/L, which is consistent with the result obtained by USEPA.