Effect of strontium-containing cross-linked sodium alginate gel on adhesion and proliferation of pre-osteoblasts
Zhang, M.; Li, H.; Xu, T.; Liu, J.; Tan, F.
Shanghai Kou Qiang Yi Xue 28(2): 123-127
2019
ISSN/ISBN: 1006-7248 PMID: 31384894 Document Number: 698028
To study the physicochemical properties of bismuth-containing cross-linked alginate gel, to observe and detect the adhesion and proliferation of mouse MC3T3-E1 cells in gluten-containing cross-linked alginate gel and provides a theoretical basis for alginate injectable tissue engineering scaffolds. Five sets of electrolytes with a final concentration of 4 g/L were prepared and cross-linked with sodium alginate solution, respectively. The compression modulus and swelling ratio of each group of gels were measured. Cells were inoculated on the surface of the composite gel, FDA/PI cells were stained to observe the adhesion morphology of the cells. Data were statistically analyzed using GraphPad Prism 6.0 software package. The swelling rate of double distilled water and PBS increased with the increase of Sr2+ concentration. The results of compression modulus showed that the strength of group C was significantly higher(P<0.05). Scanning electron microscopy showed that the pores of the composite gel increased with the increase of Sr2+ concentration. Microscopy and FDA/Pi staining showed that the cells were scattered in the initial stage of cell inoculation. The composite gel formed by mixing equal proportion of strontium ions may be used as a new type of excellent cell scaffold material in bone tissue engineering.