Cytotoxicity of 3 resin cements to human gingival fibroblasts
Zhang, C.-Y.; Niu, G.; Jin, Y.-Y.; Yu, H.; Cheng, H.
Shanghai Kou Qiang Yi Xue 26(6): 594-598
2017
ISSN/ISBN: 1006-7248 PMID: 29691552 Document Number: 691796
To study the cytotoxicity of 3 resin cements to human gingival fibroblasts (HGFs). Three resin cements (Panavia F, RelyXTM Unicem and Multilink Speed) test samples were immersed and incubated in the culture medium for 48 h at 37degrees centigrade. Cultured HGFs were exposed to two concentrations (50% and 100%) of material elutes for 24 h, 72 h and 120 h. The proliferation rate was evaluated using CCK-8 assay. The data were statistically analyzed by one-way analysis of variance using SPSS 20.0 software package. Relative growth rate(RGR) of all experimental groups ranged from 10.67% to 100.02%, the cytotoxicity grade of all groups was 0 to 4. There was no significant difference in the RGR among 3 resin cements, but the experimental group of Panavia F (uncovered with antioxidant) showed significantly lower RGR than other experimental groups. Panavia F, RelyXTM Unicem and Multilink Speed exhibit no cytotoxicity to HGFs, Panavia F(uncovered with antioxidant) shows moderate cytotoxicity.