In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHi 13 var fibroblasts
Bakopoulou, A.A.; Triviai, I.N.; Tsiftsoglou, A.S.; Garefis, P.D.
International Journal of Prosthodontics 19(1): 13-16
2006
ISSN/ISBN: 0893-2174 PMID: 16479751 Document Number: 601479
The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium. Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage. All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed. These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.