Relationship between uncoupling protein 1 gene promoter methylation and obesity in adult
Chen, Y.; Fu, X.; Qi, M.; Zhan, F.; Wang, Y.; Cai, J.; Lu, M.; Liu, L.; Ping, Z.
Wei Sheng Yan Jiu 46(1): 1-6
2017
ISSN/ISBN: 1000-8020 PMID: 29903143 Document Number: 691440
To observe the relationship between the methylation status in promoter region of uncoupling protein 1( UCP1) gene and obesity. A casecontrol study based on the hospital consisted 116 people was carried out, according to the body mass index( BMI), the subjects were divided into two groups, the overweight and obesity group with 50 samples( BMI≥24. 0) and the normal weight group with 66 samples( 18. 5≤BMI < 24), DNA samples were extracted from white blood cell and treated by hydrogen sulfite. Then mass spectrometry method was used to quantificationally detect the methylation level UCP1 gene promoter. The difference between the two groups was compared and the relationships between CpG sites and BMI were explored. Statistical analysis showed that the methylation differences between normal weight and overweight or obese group were not statistically significant, however, the CpG site UCP1-2_ Cp G_ 10. 11. 12. 13 had statistical significance in correlation coefficients with BMI according to multiple linear regression method( regression coefficient was 15. 370, P <0. 05). The UCP1 gene promoter methylation may be a factor for adult obesity.