Molecular Characteristics of the Multidrug-Resistant Mycobacterium Tuberculosis Strains in the Northwest Russia
Vyazovaya, A.A.; Mokrousov, I.V.; Zhuravlev, V.Y.; Solovieva, N.S.; Otten, T.F.; Manicheva, O.A.; Vishnevsky, B.I.; Narvskaya, O.V.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 34(1): 30-33
2016
ISSN/ISBN: 0208-0613 PMID: 27183719 Document Number: 690871
The goal of this work was to study the genotypic characteristics of the multidrug-resistant (MDR, i.e., resistant to at least rifampicine and isoniazid) Mycobacterium tuberculosis strains isolated in 2011-2012 from tuberculosis (TB) patients in the Northwest Russia. Spoligotyping of 195 M. tuberculosis isolates identified 14 different spoligotypes and assigned isolates to the genetic families Beijing (n = 162, 83%), LAM (n = 15), H3/URAL (n = 14), as well as T, Haarlem and X. Spoligotypes SIT1 (Beijing), SIT42 (LAM) and SIT262 (H3/URAL) were the most prevalent. Irrespective to the genotype, all the isolates were resistant to streptomycin. The multidrug resistance was accompanied by the resistance to ethionamide (56%), amikacin (31%), kanamycin (40%), and capreomycin (33%). The ethambutol resistance was found in 71% (n = 115) and 42% (n = 14) of the Beijing and non-Beijing strains, respectively (p < 0.05). In conclusion, the multidrug resistant M. tuberculosis population circulating in the Northwest Russia continues to be dominated by the Beijing family strains.