Expression, purification and identification of recombinant Norovirus GIi 4 capsid
Wang, J.; Han, C.; Li, N.; Xu, J.; Li, F.; Jiang, T.
Wei Sheng Yan Jiu 45(2): 252-254
2016
ISSN/ISBN: 1000-8020 PMID: 27301224 Document Number: 689609
The Norovirus GII 4 capsid protein was expressed by baculovirus expression system. The Norovirus GII 4 capsid gene was insert into the HT A plasmid. After sequencing, the recombinant plasmid was transformed into the MAX Efficiency DH10BacT competent cell to express the recombinant capsid protein of Norovirus GII 4. The recombinant capsid protein was purified by Ni-NTA His bind resin affinity chromatography purification column and was identified by Norovirus rapid test kit and SDS-PAGE. The recombinant protein was successfully expressed with the molecular weight about 60 kD, and it has good immunogenicity. The Norovirus GII 4 capsid express plasmid is constructed successfully and the recombinant capsid protein of GII 4 is got in this study.