Identification and Determination of Rupatadine and Fexofenadine by Densitometric Method
Czerwinska, K.; Wyszomirska, E.; Kublin, E.; Malanowic, E.; Mazurek, A.P.
Acta Poloniae Pharmaceutica 73(6): 1467-1474
2016
ISSN/ISBN: 0001-6837 PMID: 29634100 Document Number: 687930
Simple, precise and accurate densitometric methods were developed for the determination of two antihistamine drugs. rupatadine and fexofenadine. Silica gel 60 F₂₅₄ HPTLC plates were used as stationary phase, while mixtures of acetonitrile - water - 25% ammonia (90 : 10 : 1, v/v/v) and acetonitrile - methanol -acetate buffer at pH 5.5 (3 : 2 : 5, v/v/v) were used as mobile phases for rupatadine and fexofenadine, respectively. The detection of rupatadine and fexofenadine was conducted out at 256 and 210 nm, respectively. The limit of detection and the limit of quantification for rupatadine were found to be 0.3 and 0.1 μg/spot, respectively, and for fexofenadine, 5 and 2 μg/spot, respectively.