Correlation Analysis of the STAT3 Polymorphism and Transcription of Survivin and VEGF in Missed Abortion: Experience Among Women of the Chinese Han Population
Zhao, L.; Yang, H.
Journal of Reproductive Medicine 61(11-12): 552-556
2016
ISSN/ISBN: 0024-7758 PMID: 30226700 Document Number: 687840
To investigate the relationship between STAT3 gene polymorphism and missed abortion (MA), and the influence of STAT3 gene polymorphism on the expression of VEGF and survivin. The missed abortion group included 188 cases of MA. The control group consisted of 200 cases of surgically induced abortion in normal pregnancy. All patients were of Han ethnicity from P.R. China. STAT3 gene from patients' peripheral blood was detected using fluorescent probe real-time quantitative polymerase chain reaction (PCR), which was further analyzed to clarify genotype frequency. Survivin and VEGF mRNA levels in particular genotypes were also detected using qPCR. The STAT3 rs1053004 C/C genotype incidence in the MA group was significantly higher than that in the control group (p<0.05), while the STAT3 rs1053004 T/T and T/C genotypes showed no significant difference between the 2 groups (p>0.05). The STAT3 gene locus rs1053023 genotypes of the 2 groups were not significantly different, either (p>0.05). Furthermore, survivin and VEGF mRNA levels in the peripheral blood of the patients with STAT3 gene loci rs1053004 C/C were significantly decreased as compared to the control group (p<0.05). Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood.