Induction of transcription through the scs insulator leads to abnormal development of Drosophila melanogaster
Kyrchanova, O.V.; Leman, D.V.; Toshchakov, S.V.; Utkina, M.V.; Tikhonov, M.V.; Parshikov, A.F.; Maksimenko, O.G.; Georgiev, P.G.
Genetika 52(10): 1117-1125
2016
ISSN/ISBN: 0016-6758 PMID: 29369581 Document Number: 687159
A regulatory element named scs is one of the first insulators discovered in Drosophila, which was found on the boundary of the hsp70 domain. The 993-bp scs insulator contains two promoters at the ends and two polyadenylation signals located in the same orientation in the central part of the insulator. In the Drosophila transgenic lines, induction of a strong transcription through the scs insulator only in the direction that coincides with the direction of the two polyadenylation sites activity results in multiple phenotypic defects of the Drosophila development and embryonic lethality. A similar effect was not observed upon testing of other known Drosophila insulators.