Comparative action of peritoneal macrophage injection on immunologic responses to sheep blood red cells in normally nourished rats and in protein deficiency rats
Aschkenasy, A.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 167(2): 200-205
1973
ISSN/ISBN: 0037-9026 PMID: 4608891 Document Number: 68406
Thirty-eight Sherman rats, initially weighing less than 180 g, were divided into 5 groups, 3 of which were given a normal balanced diet and 2 a diet deficient in protein for 2 months. A week before being killed all were given an intraperitoneal injection of sheep red blood cells, 6 X 109 in 1 of the groups given the balanced diet and 3 X 109 in the other 4 groups. Of those groups, one on balanced diet and one deprived of protein were given, mixed with antigen, a suspension of 2 X 10 peritoneal macrophages taken from normally reared male rats 72 h after a glycogen injection. After death serum haemagglutinins and haemolysins were estimated. On normal diet, reduction of the dose of sheep red blood cells did not affect haemagglutinins but reduced haemolysins. The concentration of cells forming plates (CFP) and cells forming rosettes (CFR) decreased by about 50%. Addition of macrophages did not affect serum haemagglutinin and haemolysins, but CFP and CFR increased to more than control values. On the diet deficient in protein macrophages led to a fall in haemagglutinin and haemolysin, compared with values in rats on a normal diet, and also produced a fall in the concentration of CFP. When macrophages were added there was no improvement of immunological responses regarding CFP and CFR as there was with the normal diet.