The Development of Technique of Diagnostic of Vuvlvovaginal Candidiasis Based on Quantitative Multiplex Polymerase Chain Reaction
Savochkina Yu A; Rumiantseva, T.A.; Dolgova, T.I.; Guschin, A.E.
Klinicheskaia Laboratornaia Diagnostika 60(4): 56-62
2015
ISSN/ISBN: 0869-2084 PMID: 26189293 Document Number: 683414
The technique is developed including corresponding kit of reagents "AmpliSens Florocenose/Candida-FL", to be applied in laboratory diagnostic of vuvlvovaginal candidiasis. The technique is based on polymerase chain reaction in real-time and permits identifying and quantitatively estimate in biological material content of five most prevalent agents of candidiasis: Candida albicans, C. glabrata, C. krusei, C. paprapsilosis, C. tropicalis. The experimental determination of analytical characteristics of developed technique was carried out. The analytical sensitivity amounted to 100 GE/mL (genome equivalent in 1 mL), linear range -from 200 to 2 x 10 7 GE/mL for every specie of detected Candida. The comparison of quantitative results of detection of content of Candida spp using the developed technique with results of inoculation demonstrated their mutual matching. In logarithm presentation the relation coefficient of lg (GE/mL) to lg (CFU/mL) amounted to 1.2. The correlation of results characterized by coefficient R 2 equal 0.76.