Multiplex-polymerase chain reaction assay for the authentication of the mackerel Scomber colias in commercial canned products
Infante, C.; Manchado, M.
Journal - Association of Official Analytical Chemists 89(3): 708-711
2006
ISSN/ISBN: 0004-5756 PMID: 16792069 Document Number: 599878
A multiplex-polymerase chain reaction (PCR) system was developed for the authentication of the mackerel Scomber colias in commercial canned products. This novel method consists of an S. colias-specific fragment [159 base pairs (bp)] located in the nontranscribed spacer (NTS) sequence, and a Scomber genus-specific PCR product in the 5S rRNA gene (196-201 bp) as a positive amplification control. The system was assayed using 18 different canned products labeled as S. colias. A positive identification was made in all but one sample, revealing this methodology as a potential molecular tool for direct application in the authentication of S. colias canned products.
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