Shifts in Urine Protein Profile during Dry Immersion
Pastushkova L Kh; Kononikhin, A.S.; Tiys, E.S.; Nosovsky, A.M.; Dobrokhotov, I.V.; Ivanisenko, V.A.; Nikolaev, E.N.; Novoselova, N.M.; Custaud, M.A.; Larina, I.M.
Aviakosmicheskaia i Ekologicheskaia Meditsina 49(4): 15-19
2015
ISSN/ISBN: 0233-528X PMID: 26554129 Document Number: 682236
The study was aimed at tracking the proteomic profile of urine in 8 normal volunteers to 5-day dry immersion (DI). The proteome composition was determined by chromatography-mass spectrometry on high-efficient on-line liquid nano chromatograph Agilent 1100; complementary information about the protein spectra was obtained by dint of mass-spectrometer MaXis Impact 4G and hybrid mass-spectrometer LTQ-FT. Functional associations between proteins and biological functions were analyzed using computer system ANDCell (Associative Networks Discovery in Cells). A total of 256 proteins were identified; for 43 proteins difference in the detection rate during the baseline data collection and on DI day 4 exceeded 20%.