The effect of estrogen on adipogenic differentiation of rat bone mesenchymal stem cells
Wang, J.; Ouyang, N.-j.; Zhang, P.; Dai, Q.-g.; Fang, B.; Jiang, L.-y.
Shanghai Kou Qiang Yi Xue 24(5): 519-524
2015
ISSN/ISBN: 1006-7248 PMID: 26598181 Document Number: 680865
In this study, 10⁻⁹ mol/L 17 β-estradiol (E2) was applied in the adipogenic differentiation of rat bone mesenchymal stem cells (rBMSCs) and the effect of E2 was explored. Rat BMSCs were obtained from the femurs and tibias of SD rats. 10⁻⁹ mol/L E2 was involved in the adipogenic differentiation of rBMSCs. Oil red staining, real time PCR and Western blot were carried out to detect the effect of 10⁻⁹ mol/L E2 on adipogenic differentiation of rBMSCs. The data was statistically analyzed using SPSS 19.0 software package. The use of 10⁻⁹ mol/L E2 decreased the amount of lipid droplets in rBMSCs and weakened the expression of adipogenic related genes and proteins like C/EBP α, C/EBP β, PPAR γ, aP2, and ARDP, which were significantly lower than the adipogenic induced group. The use of 10⁻⁹ mol/L E2 inhibited adipogenic differentiation of rBMSCs significantly in vitro.