DNA Extraction of Cast-off Cells of Fingerprints from 502 Glue Fumigated Contact Samples
Wang, X.-w.; Leng, X.-f.; Wang, S.-y.
Fa Yi Xue Za Zhi 31(6): 454-455; 461
2015
ISSN/ISBN: 1004-5619 PMID: 27141804 Document Number: 679989
To establish a method of fingerprint position, sample transfer and fingerprint DNA extraction in contact samples. Sixty-six cases were visualized by 502 glue fingerprint fumigation. Two methods, ordinary wipe and acetone wipe, were used to transfer cast-off cells of fingerprints from testing samples, respectively. DNA was extracted and purified by ultramicro magnetic bead kit. The data was resolved on genetic analysis after amplification. In 33 samples, 30 samples got better STR analysis by acetone wipe method. The peak range was 1,000-4,000 RFU and peak shapes were equable. It was hard to get ideal STR typing by ordinary wipe method. The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method. The method shows better STR analysis result, which might be a better method for forensic science practice.