Comparative characteristics of HLA system gene distribution in patients with lung tuberculosis of the Russian population of Chelyabinsk region
Burmistrova, A.L.; Beliaeva, S.V.; Suslova, T.A.; Bukharin, O.V.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 35-41
2014
ISSN/ISBN: 0372-9311 PMID: 25286510 Document Number: 676832
Study of distribution of genes and HLA system haplotypes A, B, DRB1, DQA1, DQB1 in healthy individuals and patients with lung tuberculosis, members of the Russian population of Chelyabinsk region for isolation of risk markers for the development of various forms of tuberculosis. The study group consisted of 86 patients with lung tuberculosis of the Tuberculosis Dispensary No. 3 of Chelyabinsk of Russian nationality. 239 healthy donors of Chelyabinsk Hemotransfusion Station of Russian nationality composed the comparison group. HLA-typing was carried out by multi-primer polymerase chain reaction--PCR SSP, HLA II genotyping--by DNA technology (Russia) kits, HLA class I--by methods described in the article by Downing J.M.G. et al. (2004). Result detection was carried out by electrophoresis. In lung tuberculosis patients high frequency of detection of HLA haplotype DRB1*16-DQA1*01:02-DQB1*05:02/4 was established. In patients with fibrous-cavernous form B*08 and DRB1*03 gene frequency of detection increased and DRB1*07 and DQA1*02:01 genes did not occur. During focal form high frequency of HLA B*15 and HLA DRB1*15 alleles was determined compared with infiltrative form. HLA A*01-B*08-DRB1*03-DQA1*05:01-DQB1*02:01 haplotype only occurred ingroups of patients with more severe forms of tuberculosis. Markers of sensitivity to clinical phenotypes of tuberculosis were isolated.