Research on expression of somatomedin b domain of proteoglycan 4 and recombinant protein aggregation
Wang, L.; Han, Z.; Chen, W.; Du, P.; Sun, A.; Yang, P.; Zhao, H.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 31(6): 1319-1324
2014
ISSN/ISBN: 1001-5515 PMID: 25868252 Document Number: 675756
Recombinant protein SMB(PRG4) containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMB(PRG4), with high-level expression was purified. By using size-exclusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to dimeric form. Structure prediction and non-reducing electrophoresis revealed that SMB(PRG4), was a non-covalently bonded dimer.