Expression of EIIIA+ fibronectin in incised wound of rat's skin
He, M.; Zhao, H.; Shen, Y.-w.; Xue, A.-m.; Chen, L.; Gu, Y.-j.; Zhao, Z.-q.
Fa Yi Xue Za Zhi 30(6): 409-412
2014
ISSN/ISBN: 1004-5619 PMID: 25816567 Document Number: 674110
To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat's skin and injury time. The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin samples were taken at the margin of wound. The expression of the EIIIA? fibronectin was detected by immunohistochemistry and Western blotting and the relationship be- tween its expression and injury time was observed. Results The expression of EIIIA+ fibronectin was not observed immediately. The basal cell of skin began to show positive expression 0.5 h after injury. With the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the Western blotting analysis. The expression of EIIIA+ fibronectin could be used for estimation of injury time in the early stage of skin injury.