Optimization of enzymatic saccharification of microwave pretreated sugarcane tops through response surface methodology for biofuel
Maurya, D.P.; Vats, S.; Rai, S.; Negi, S.
Indian Journal of Experimental Biology 51(11): 992-996
2013
ISSN/ISBN: 0019-5189 PMID: 24416936 Document Number: 665970
The optimization of biomass loading enzyme loading, surfactant concentration and incubation time, using response surface methodology (RSM) and Box Behnken design for enzymatic saccharification of sugarcane tops (SCT) for maximum recovery of fermentable sugars using crude cellulases, resulted in 90.24% saccharification efficiency. Maximum saccharification yield of 0.376 g/g glucose as substrate for ethanol production was observed at optimal conditions of 10% biomass loading (pretreated), 100FPU/g of cellulase loading, 0.04% (w/w) surfactant concentration and 72 h of incubation time.