Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus
Zhao, X.-P.; Ge, Z.-W.; Zhang, Y.-F.; Lan, X.-H.; Zhang, B.-L.
Zhongguo Zhong Yao Za Zhi 38(11): 1820-1822
2013
ISSN/ISBN: 1001-5302 PMID: 24010303 Document Number: 665652
Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.