Detection of Salmonella, Shigella and Staphylococcus aureus based on quantum dots and immunomagnetic beads
Li, Q.; Chen, P.; Wang, J.; Zhang, S.; Yan, J.
Wei Sheng Yan Jiu 42(4): 660-663
2013
ISSN/ISBN: 1000-8020 PMID: 24024384 Document Number: 665378
To establish a detecting method of Salmonella, Shigella and Staphylococcus aureus by using quantum dots and immunomagnetic beads. The polychrome colour CdSe quantum dots were synthetized by water phase process. Triad colour quantum dots were conjugated with specific anti-Salmonella, anti-Shigella and anti-Staphylococcus aureus antibody by covalent cross-linking reaction, and then the coupled product was confirmed by SDS-PAGE and ultraviolet absorption spectrum. Using immunomagnetic enrichment and quantum dots labeling specific antibody, the fluorescence intensity of Salmonella, Shigella and Staphylococcus aureus were observed by fluorescence microscope. The coupled reaction was successful by SDS-PAGE and ultraviolet absorption spectrum. Using immunomagnetic enrichment and quantum dots with different color and different wavelength labeling specific antibody, the detection time was less than 2h in the same reaction system. The samples infection with target bacteria were detected directly. The detection limit was 10 CFU/ml. This method can detect Salmonella, Shigella and Staphylococcus aureus quickly and specificly.