Identification and characterization of enterovirus 71 isolated in Yunnan province
Zhao, S.-W.; Yin, W.-D.; Cun, J.-P.; Gao, Q.; Yin, J.; Liu, G.-J.; Xu, W.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 27(4): 263-265
2013
ISSN/ISBN: 1003-9279 PMID: 24579470 Document Number: 665097
Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province. Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera. All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000. Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.