Random amplified polymorphic DNA identification of six Bactrocera (Diptera: Tephritidae) species in Yunnan Province of southwest China
Zhang, L.; Zhang, Z-ying.
Ying Yong Sheng Tai Xue Bao 18(5): 1163-1166
2007
ISSN/ISBN: 1001-9332 PMID: 17650876 Document Number: 608353
In this paper, the genetic relationships among six fruit fly species of Bactrocera in Yunnan Province of Southwest China, i. e., B. tau, B. scutellaris, B. scutellata, B. cucurbitae, B. dorsalis and B. correcta, were studied by using the technique of random amplified polymorphic DNA (RAPD). Five primers with good repetition and high polymorphism were screened from 131 primers, and a total of 302 bands were amplified, among which, I I I bands were of genetic polymorphism. The primers OPC-01, OPI-17, OPL-07, OPL-08 and OPL-16 could be used for the identification of the 6 fruit fly species.