Effects of long-term intervention of moxa smoke on T lymphocyte subsets and CD4+ CD25+ Treg in peripheral blood of Wistar rats
Liu, P.; Pan, X.-J.; Han, L.; Yang, J.; Hu, H.; Cai, H.; Li, R.-X.; Zhu, M.-X.; Yang, Z.-H.; Zhao, B.-X.
Zhongguo Zhen Jiu 33(2): 145-148
2013
ISSN/ISBN: 0255-2930 PMID: 23620944 Document Number: 664352
To investigate the cellular immune regulation of the long-term intervention of moxa smoke. Thirty-two Wistar rats were randomly divided into a blank group, a low concentration group, a medium concentration group and a high concentration group, 8 cases in each group. In addition to the blank group, rats in the other groups were exposed to the corresponding concentration moxa smoke for 20 min every day, the T lymphocyte subsets and proportion of the CD4+ CD25+ Treg in CD4+ T cells in peripheral blood were tested by flow cytometry after 6 months. Compared with the blank group, the proportions of CD3+ CD4+, CD3+ CD8+ T cells and CD3+ CD4/CD3+ CD8+ in the other 3 moxa smoke groups were not significantly different (P > 0.05), while the proportions of the CD4+ CD25+ Treg in CD4+ T cells were significantly lower (P < 0.05), but no statistically significant differences among those 3 moxa smoke intervention groups (P > 0.05). Long-term moxa smoke intervention has no significant effect on the proportions of CD3+ CD4+, CD3+ CD8+ T cells and CD3+ CD4+/CD3+ CD8+, but it can decrease the proportions of the CD4+ CD25+ Treg in CD4+ T cells in peripheral blood of rats. The way produced by pretreatment with moxa smoke may play immunomodulatory effect.