Improvement of carbon tetrachloride drug-induced liver injury model in vitro
Wu, H.-J.; Gong, X.; Yang, Y.-T.; Wang, Y.-Z.; Li, X.; Xin, H.; Hu, L.-K.
Zhongguo Zhong Yao Za Zhi 37(23): 3633-3636
2012
ISSN/ISBN: 1001-5302 PMID: 23477154 Document Number: 661840
To establish an ideal CCl4 drug-induced liver injury model in vitro. Traditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase (ALT) in supernatant was detected by biochemical method. 4-Methyl-tetrazolium (MTT) chromatometry was adopted for determining cell activity. The improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect. The improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies.