HPLC determination of hydroxysafflor yellow a in Mongolian medicine Dedu Honghuaqiwei pill
Chen, C.-L.; Hu, S.-L.
Zhongguo Zhong Yao Za Zhi 37(23): 3673-3675
2012
ISSN/ISBN: 1001-5302 PMID: 23477163 Document Number: 660712
To establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill. The determination was performed by HPLC method on Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26: 2: 72) as mobile phase. The column temperature was 30 degrees C and the flow rate was 1.0 mL x min(-1). The linear rang of hydroxysafflor yellow A was 0.068-0.408 microg and the recovery was 97.66%. The result is accurate with good resolution, and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.