Primary establishment of an alphaLISA assay for detection of HAV IgM
Gan, X.; Wang, Y.; Song, J.; Cui, Y.; Sun, P.; Gao, C.; Li, C.-P.; Han, J.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 26(2): 139-141
2012
ISSN/ISBN: 1003-9279 PMID: 23002558 Document Number: 660468
To develop an AlphaLISA method for detection of antibody of Hepatitis A virus. After hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, acceptor bead concentration have been explored and determined by both dot-blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method. The sensitivity and specificity for anti-HAV IgM were 78% and 98.5%, the positive and negative predictive value were 95% and 93%. A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.