Mutation analysis of the HBV reverse transcriptase in nucleos(t)ide-treated patients with chronic HBV infection

Jiao, X.-J.; Peng, X.; Jiao, X.-M.; Wang, J.-S.; Sun, X.-W.; Zhao, P.-L.; Wang, S.-Y.; Liu, J.-Q.; Li, T.; Yang, J.-X.; Jiao, X.-J; Peng, X; Jiao, X.-M; Wang, J.-S; Sun, X.-W; Zhao, P.-L; Wang, S.-Y; Liu, J.-Q; Li, T; Yang, J.-X

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Mutation analysis of the HBV reverse transcriptase in nucleos (t) ide-treated patients with chronic HBV infection

Jiao, X.-J.; Peng, X.; Jiao, X.-M.; Wang, J.-S.; Sun, X.-W.; Zhao, P.-L.; Wang, S.-Y.; Liu, J.-Q.; Li, T.; Yang, J.-X.

Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 26(6): 453-455

2012

ISSN/ISBN: 1003-9279

PMID: 23627028

Document Number: 660064

To characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment. Serum samples of 229 CHB patients with NA treatment were obtained. Full-length HBV RT sequences were amplified, sequenced and analyzed, on the following NA resistant (NAr) mutations belonging to different NAr pathways. Among 229 HBV isolates, 14.41% (33/229) and 85.59% (196/229) were genotype B and C, respectively; and the patients with HBV genotype C may be more susceptible to develope resistant mutations than patients with HBV genotype B(chi2 = 2.95, P < 0.05). NAr mutations were detected in 63 CHB patients. Mutations were not found at rtI169, rtT184, rtA194 or rtS202. RtM204 mutations were detected at the highest frequency among 63 mutants (40/63, 63.49%) and found to display 11 combination mutation patterns, in which rtM204I were associated with rtL80I/V and rtL180M, and rtM204V were associated with rtL1l80M, respectively. Conclusions There are complicated mutation patterns in the HBV RT region for chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment. RtM204V/I mutation was the highest.