Change of proinflammatory cytokine profile in human intestine in dysbacteriosis caused by the antibiotics therapy
Aleshukina, A.V.; Iagovkin, É.A.; Bondarenko, V.M.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2012(6): 81-85
2012
ISSN/ISBN: 0372-9311 PMID: 23297638 Document Number: 657163
Study of the proinflammatory cytokines level in human gut of adults with intestinal dysbacterioses (ID). It is surveyed the out-patient 110 adult with torpid clinical form of enteric infection. All of patients received antibiotics of a wide spectrum of action. Patients were randomized into two groups by 55 in each. Studying of structure of gut microflora with use of differential-diagnostic media and criteria of an estimation dysbiotic infringements according to Russian Federation Standard "Protocol of patient management. Intestine dysbacterioses" (2003). Also defined quantity of IL-1beta, IL-6 and INF-gamma in coprofiltrates by ELISA ("Vector-Best", Russia). By comparison of positive tests proinflammatory cytokines in coprofiltratis and the gut dysbiosis expression (ID) it was revealed that the most adverse variant of revealing IL-6 in any combinations with tested cytokines in 57,2 +/- 4,7% cases. Similar cases were characterized subcompensated and decompensated gut dysbacteriosis in 65 +/- 4,5% and 100% of cases. Compensated (subclinical) form of ID revealed at patients, in coprofiltratis which INF-gamma was detected without of combinations with IL-6. Testing of proinflammatory cytokines in coprofiltrates of individuals in ID may be one of the prognosis criteria of inflammatory reactions course duration and evaluation of therapeutic effect of disordered microbiocenosis correction.