Identification of catalytically active groups of pea (Pisum sativum L.) beta-glucosidase
Ershova, A.N.; Barkalova, O.N.
Prikladnaia Biokhimiia i Mikrobiologiia 47(3): 259-264
2011
ISSN/ISBN: 0555-1099 PMID: 21790023 Document Number: 652310
Functional groups ofcytoplasmic pea beta-glucosidase pretreated to an electrophoretically homogeneous state were identified. Data on the pH dependence of the enzyme activity, calculated heat of ionization, photoinactivation of the enzyme in the presence of methylene blue, and inactivation of the enzyme with diethyl pyrocarbonate suggest that the catalytic site of beta-glucosidase contains the carboxyl group of glutamic or aspartic acids and the imidazole group of histidine.