Comparison of the mechanism of cardiotonic action of the fraction 3 of MAI-BAO and digoxin on rat isolated heart
Yapo, A.F.; Yapi, H.F.; Bidie, A.d.P.; Abo, K.J.-C.; Otokore, D.A.; Datte, Y.J.; Djaman, A.J.
Le Mali Medical 26(2): 49-54
2011
ISSN/ISBN: 1993-0836 PMID: 22765949 Document Number: 651590
MAI-BAO (MB) is a beverage obtained by fermentation of sweet Camellia sinensis tea by the Tea-Mushroom treasure. The action of the chromatographic fraction 3 of MAI-BAO (MB-F3) is cardiotonic. To compare the mechanism of cardiotonic activity of MB-F3 and digoxin (Dx) in rats. MB-F3 obtained by gel chromatography on sephadex G-50 and digoxin are used to infuse the isolated rat heart with the device Langendhorff for the record of cardiac contractions. The biochemical mechanism of cardiac effect of MB-F3 and Dx was determined by measuring phosphorus by the method of Sumner. MB-F3 has a significant positive inotropic effect (P<0.05) as Dx (P <0.01) with respective effective Doses 50% 1mg/ml and 10-6 mg/ml. Furthermore, MB-F3 exerts a competitive inhibition on the ATPase-Na+/K+ less than Dx (P<0.05). MB-F3 exerted digitalis-like effect less than digoxin on the rat heart.