Technical strategies in the research of plant salt-responsive proteomics: a review
Zhang, H.; Dai, S.-j.
Ying Yong Sheng Tai Xue Bao 22(8): 2201-2210
2011
ISSN/ISBN: 1001-9332 PMID: 22097388 Document Number: 650130
Soil salinization is one of the key factors limiting plant growth and distribution. To explore plant molecular salt-responsive mechanisms is the basis of enhancing plant salt tolerance in virtue of molecular biological techniques, and also, the hotspot of plant physiology and molecular ecology. High throughput proteomics approaches include two-dimensional electrophoresis (2-DE), blue-native/SDS-PAGE (BN/SDS-PAGE), two-dimensional fluorescence difference gel electrophoresis (2D DIGE), liquid chromatography (LC), and various mass spectrometry (MS) techniques, being widely applied in the research of plant salt-response and supplied important information for understanding plant molecular salt-tolerant mechanisms. In this paper, a review was made on the technical strategies applied in the research of plant salt-responsive proteomics.