Experimental research regarding the influence of the stress factors in an animal model of HELLP syndrome
Diaconu, M.; Diaconu, C.; Tarţău, L.; Lupuşoru, C.ăt.ăl.-E.
Revista Medico-Chirurgicala a Societatii de Medici Si Naturalisti Din Iasi 115(3): 876-880
2011
ISSN/ISBN: 0048-7848 PMID: 22046802 Document Number: 649411
To study the effects of licht/dark induced-stress in an animal model of HELLP syndrome. The experiment was carried out on white female Wistar rats treated intraperitoneally, for 4 weeks, as follows: Group 1: saline solution 0.5ml/100g weight; Group 2: saline solution + dark induced-stress, continuously, 28 days; Group 3: saline solution + light induced-stress, continuously, 28 days; Group 4: endotelin-l; Group 5: endotelin-1 + dark induced-stress, continuously, 28 days; Group 6: endotelin-1 + light induced-stress, continuously, 28 days. Endotelin-1 0.125nmol/kbw was initially administered, followed by 0.25nmol/kbw 30 min later, 2 times/week. At the end of the experiment blood pressure was measured and blood samples were taken to assess: leukocyte formula, phagocytic capacity of peripheral neutrophils and serum complement activity, aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT) and lactic dehydrogenase (LDH) activity, total bilirubin and lipid profile. In these experimental conditions we obtained an animal model of partial HELLP syndrome (elevated liver enzyme levels and a low platelet count, values emphasized by light induced-stress, without hemolysis). In chronically stressed animals we found the highest levels of blood pressure, a decrease of plasmatic cortisol level and of phagocytic capacity of peripheral neutrophils and an increase of total cholesterol.