Development and evaluation of a serological protocol of fluorescence polarization for the preliminary study of Brucella spp antibodies in humans
Sánchez-Villalobos, A.; Urdaneta-Fernández, M.; Rubio-Fuenmayor, E.Ã; Molero-Saras, G.; Luzardo-Charris, C.; Corona-Mengual, C.
Investigacion Clinica 52(1): 48-57
2011
ISSN/ISBN: 0535-5133 PMID: 21614813 Document Number: 648234
In order to show the development and scope of a serological analysis method based on fluorescence polarization assay (FPA) from a drop of blood obtained by the capillary technique, a Brucella antibody assay was performed on a group of 321 high-risk workers. The results were compared with data from the analysis of blood serum by FPA and a competitive enzyme immunoassay (ELISA-c). The number of concordance was 318 (99.06%), and discordant 3 (0.93%), which were negative in serum by fluorescence polarization (FPAs) and ELISA-c, but positive with capillary FPA (FPAc). The comparative results FPAc were: sensitivity 100%; specificity: 99.05%; positive predictive value 66.67%; negative predictive value 100.0%; false positive rate: 0.95%; false negative rate: 0%; accuracy: 98.0%; odds ratio: 203.00. The youden J for both FPA methods was 0.667. The identification was considered reliable and the correlation of both procedures, FPA and ELISA-c, was no statistically different (P > 0.05%), which allows to highly recommend the study implementation of human brucellosis with capillary blood as a preliminary method.