Fluorescence polarization immunoassay to determine aminoglycoside modifying enzymes activity
Pagani, L.; Perduca, M.; Mills, V.; Romero, E.
Microbiologica 9(4): 423-430
1986
ISSN/ISBN: 0391-5352 PMID: 3022111 Document Number: 280777
The possible use of polarized-immunofluorescence to evaluate aminoglycoside-modifying enzyme activities has been investigated. The TDX instrument was used to determine the residual quantity of aminoglycoside following inactivation during the assay procedure. Through the spectrum of the modified aminoglycoside antibiotics the enzymes encoded on eleven R-factors from clinical isolates of Enterobacteriaceae have been identified.