Semiquantitative microbiological residue method for hygromycin B in chicken eggs
Begue, W.J.; Kline, R.M.
Journal - Association of Official Analytical Chemists 56(1): 20-22
1973
ISSN/ISBN: 0004-5756 PMID: 4592490 Document Number: 64755
A method recently has been developed for the detection of hygromycin B residues in chicken eggs at the 15 ppb level. Protein is precipitated with trichloroacetic acid. After centrifugation, the supernatant liquid is adjusted to pH 7.0 and placed on an IRC-50 ion exchange column. The column is washed with water and then acetone, and subsequently the hygromycin B is eluted with ammonium hydroxide. The eluate is evaporated to dryness and reconstituted in a known amount of water; hygromycin B is microbiologically detected with Pseudomonas syringae by bioautography on thin layer chromatographic plates.