Cellular aspects of regulation of nuclear factor kappaB (NF-kappabeta) transcriptional activity in sensory neurons in vitro
Gushchina, S.V.; Volkova, O.V.; Krugliakov, P.P.; Magoulas, K.B.
Morfologiia 137(3): 22-26
2010
ISSN/ISBN: 1026-3543 PMID: 20960709 Document Number: 646652
Nuclear factor kappaB (NF-kappaB) controls the expression of multiple anti/proapoptotic genes, including those in the nervous cells. Intranuclear deacetylation and acetylation events are implicated in the regulation of NF-kappaB transcriptional activity. The goal of the work was to demonstrate the transcriptional activity of NF-kappaB in the sensory neurons in vitro and to find out whether NF-kappaB activity could be controlled by modification of acetylation processes, using a transgenic line of NF-kappaB reporter mice in which activation of NF-kappaB drives the expression of the lac-z gene. We show that the expression of the reporter gene was absent in transgenic neurons cultured in normal growth medium or stimulated by TNF-alpha. However, NF-kappaB transcriptional activity in most neurons was induced by in vitro exposure to Trichostatin A, which is a specific inhibitor of histone deacetylases. Taken together our results show that the transcriptional activity of NF-kappaB is limited in sensory neurons due to a transcriptional repression mechanism mediated by histone deacetylases.